Journal: bioRxiv
Article Title: Tools for Genetic Engineering and Gene Expression Control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides
doi: 10.1101/2023.08.25.554875
Figure Lengend Snippet: A: Schematic of a mating scheme for transfer of the Tn 7 transposon and Tn 7 transposase-expressing plasmid into the recipient of interest. B: Schematic of a mating scheme for delivery of a Tn 7 transposon into R. sphaeroides constitutively expressed with plasmid-encoded Tn 7 transposase genes ( tnsABCD ). C&D: Transposition efficiency into N. aromaticivorans and R. sphaeroides, respectively, using different mating schemes. E: Growth of N. aromaticivorans strains with or without att::Tn 7 insertions in rich (464a) and minimal (SIS+glucose) media. F: Growth of R. sphaeroides strains with or without att::Tn 7 insertions in rich (LB) and minimal (SIS) media. G: Stability of a kanR Tn 7 insertion in both N. aromaticivorans and R. sphaeroides following serial passaging in the absence of selection. N.a.=N. aromaticivorans , R.sp. = R. sphaeroides . Summary statistics and growth curve data can be found in tables S2-S5.
Article Snippet: N. aromaticivorans strains were grown in 464a (5g tryptone, 5g yeast extract, 1g glucose per liter) (DSMZ) or SIS supplemented with 1% glucose.
Techniques: Expressing, Plasmid Preparation, Passaging, Selection